Effects of peripartal rumen-derived direct-fed microbial supplementation on lactation performance, metabolism, ruminal fermentation, and microbial abundance in dairy cows

The objective of this study was to evaluate the effects of a rumen-derived direct-fed microbial (DFM) product on performance, blood biomarkers, ruminal fermentation, and bacterial abundance in dairy cows during the transition period until 100 DIM. Fifty-six Holstein cows were enrolled in a randomized complete block design from -21 to 100 DIM. Cows were blocked based on expected calving date, parity, and previous lactation milk yield for multiparous cows or genetic merit for primiparous cows. At -21 DIM, cows were randomly assigned to either a basal control diet supplemented with 150 g/d ground corn (CON; n = 29) or the control diet supplemented with ground corn plus a rumen-derived DFM product (GF; n = 27, 150 g/d ground corn + 5g/d of Galaxis Frontier [Native Microbials, San Diego, CA]; Clostridium beijerinckii at 1.0 × 10⁷ cfu; Pichia kudriavzevii at 1.0 × 10⁸ cfu; Ruminococcus bovis at 1.0 × 10⁸ cfu; Butyrivibrio fibrisolvens at 1.0 × 10⁸ cfu) that was top-dressed once a day. All cows received the same basal close-up diet from -21 DIM until calving (1.56 Mcal/kg DM and 14.46% CP) and the same lactation diet from calving to 100 DIM (1.76 Mcal/kg DM and 15.69% CP). We collected blood samples to measure biomarkers of metabolism, inflammation, and oxidative stress, as well as rumen fluid via esophageal tubing for ammonia, VFA, and microbial abundance from a subset of multiparous cows (n = 12/treatment) at various time points from -22 to 100 DIM. Compared with CON, GF cows produced more milk (+4.1 kg/d) during the postfresh period (6-14 wk). However, GF cows tended to produce more milk (+2.9 kg/d) than CON during the entire trial (0-14 wk). Although DMI was not affected by treatment, GF cows had greater feed efficiency (+0.18, milk/DMI) in the postfresh period. Compared with CON, GF cows had lower blood plasma glucose and higher BHB. Blood biomarkers showed greater concentrations of ceruloplasmin, haptoglobin, and reactive oxygen metabolites (ROM) in GF cows compared with CON. Compared with CON, GF cows had greater ruminal molar proportions of butyrate and tended to have greater valerate and lower acetate. These changes in ruminal VFA were coupled with alterations in ruminal microbial abundance, where compared with CON, GF cows tended to have a greater abundance of lactate-utilizing species (Megasphaera elsdenii), but lower abundance of cellulose-utilizing species (Fibrobacter succinogenes). Although greater ROM was accompanied by a mild inflammatory condition in GF cows, this was not detrimental to milk yield and DMI. Overall, our results suggest that supplementing GF in the transition period until 100 DIM positively affects lactation performance.