Preparation, characterization, applications and analytical studies of some new azo dyes derived from sulfanilamide

Background: Azo compounds are a class of organic molecules characterized by the presence of a double bond between two nitrogen atoms and contain one or more azo functional groups. Aim of study: This study involves the preparation of two new azo dyes from Sulfanilamide with 8-hydroxyquinoline (L1)[4-((8-hydroxyquinolin-5-yl) diazenyl) benzenesulfonamide] and Vanillic acid (L2)[ 4-hydroxy-3-methoxy-5-((4-sulfamoylphenyl) diazenyl)benzoic acid]. C.H.N., 1H-NMR, FT-IR, and visible spectroscopic techniques have described the characterization of dyes. Results: The electronic spectra of these azo dyes were studied in terms of acid-base properties at different pH values (2-12), which includes establishing isobestic points and determination of protonation and ionization constants. The impact of various polarity solvents on the electronic spectra was the subject of the other investigation. The biological effectiveness were also studied with two types of bacteria (Escherichia Coli and Staphylococcus Aureus) and two types of fungi (Candida albicans and Aspergillus niger). The Azo compounds solutions (L1 and L2) were used to determine the nitrite ion by forming the two dyes and a study of the precision and accuracy was investigated. The ability to use the two dyes as acid-base indicators.