Phenotypic Detection and Antibiotic Susceptibility of Methicillin Resistant Staphylococcus aureus (MRSA) in Clinical Isolates at Maternal & Child Tertiary Care Hospital-Western Rajasthan

Methicillin Resistant Stahylococcus aureus (MRSA) continue to be a major nosocomial pathogen causing increased morbidity and mortality worldwide. Emergence of hospital acquired MRSA strain posses great challenge for treatment leading to higher morbidity and mortality. This was a retrospective study done in a tertiary care hospital from November 2022 to July 2023. Various clinical samples such as pus, blood, CSF, vaginal swab, pleural aspirate were received & processed and Staphylococcus aureus species were identified as per standard laboratory protocol. AST was performed & MRSA was detected by disc diffusion test as per CLSI guideline 2022. Total 214 Staphylococcus aureus were isolated. 142 were MRSA, 72 were MSSA. Maximum number of Staphylococcus aureus (both MRSA & MSSA) were isolated from blood (48.13%) followed by pus (42.05%). MSSA were found 100% sensitive to Amikacin, & Lenezolid followed by Cotrimoxazol (97.22%), Piperacillin –tazobactam (97.22%), gentamicin (95.83%). MRSA strain 100% sensitive to Linezolid followed by Amikacin (95.77%), Gentamicin (91.54%) and Ciprofloxacin (72.53%). The result showed that Staphylococcus aureus isolated were resistant to most of the commonly used antibiotics. MRSA isolates were found sensitive to Linezolid, Amikacin and ciprofloxacin. There is need to establish antimicrobial surveillance system which will help in making antibiotic policy to prevent overuse and misuse of antibiotics.