Low-frequency Raman spectroscopy as a new tool for understanding the behaviour of ionisable compounds in dispersed mesophases
The intensity of the VDOS signal of MO + OA mesophases scaled with phase-specific transformations, such as from the bi-continuous cubic Im3¯m phase (V<sub>2</sub>) or lamellar-based vesicles to the reversed hexagonal p6m phase (H<sub>2</sub>). For NG subtle changes in the lattice parameter of the V<sub>2</sub> phase of NG + MO mesophases coincided with the apparent dissociation constant (pK<sub>a</sub><sup>app</sup>) of NG, however, slight variations between the pK<sub>a</sub><sup>app</sup> of NG determined by equilibrated samples analysed using SAXS and non-equilibrated samples analysed using LFR suggest structural hysteresis upon changes in the protonation state of NG. This approach offers an efficient method for studying the phase behaviour of lipid systems under varying pH and potentially other conditions such as temperature.
Authors
- Kārlis Bērziņš (ORCID: https://orcid.org/0000-0001-6545-5522)
- Ben J. Boyd (ORCID: https://orcid.org/0000-0001-5434-590X)
- Pablo Mota‐Santiago (ORCID: https://orcid.org/0000-0002-1537-4318)
- Vito Foderà (ORCID: https://orcid.org/0000-0003-2855-0568)
- Shinji Kihara (ORCID: https://orcid.org/0000-0001-9753-4217)
- L Krog (ORCID: https://orcid.org/0000-0001-9444-1517)
Institutions
- University of Copenhagen (DK)
- Lund University (SE)
- MAX IV Laboratory (SE)
Publication Details
- Journal
- Journal of Colloid and Interface Science
- Published
- 2024-12-07
- DOI
- https://doi.org/10.1016/j.jcis.2024.12.032
- Citations
- 5
- Primary Topic
- Analytical Chemistry and Chromatography
- Type
- article
- Field-Weighted Citation Impact
- 1.20
Funders
- Københavns Universitet
- Novo Nordisk
- Novo Nordisk Fonden