Evaluation of the antifungal and antibacterial activities of plant extracts of the genera Centaurea papposa red and Punica granatum L belonging to the family Asteraceae and Lythraceae

Centaurea papposa and Punica granatum L are plants belonging to the Acteracea and Punicacea families, respectively, and their richness in secondary metabolites led us to extract the leaves using methanol; the methanolic extract is then fractionated using solvents of increasing polarity. Based on these data, we were interested in the biological study and evaluation of two biological activities of these plants. Firstly, we studied the antibacterial activity on five bacterial strains (Bacillus subtilis, staphylococcus aureus, Esherichia coli, Proteus vulgaris, Pseudomenas aeruginosa). This activity was carried out using the diffusion method on disks impregnated with different concentrations of the extract. The results show that the dichloromethane extract of Centaurea papposa is the most active on the strains tested, with a maximum inhibition diameter of 15 mm for the initial concentration (MS) against Proteus vulgaris. Meanwhile, the n-butanol extract of Punica granatum L has the best antibacterial power against staphylococcus aureus with a diameter of 26.5 mm. On the other hand, we evaluated the antifungal activity on three fungal strains (Fusarium culmorum, Fusarium oxysporum, Fusarium cerealis) by the well diffusion method and the results show that the strains are very sensitive to three extracts with significant areas of inhibition.