ITS- 1 gene based PCR as a diagnostic tool for phylogeographic characterization of Trypanosoma evansi in naturally infected equines in agro-climatic zones of Uttar Pradesh, India

Background: Six representative ITS1-PCR amplified products were used for the amplifications of conserved regions of 18S and 5.8S rDNA to amplify Internal Transcribed Spacer1 (ITS1) gene of 540 bp of Typanosoma evansi isolates in equine from three agro-climatic zones of Eastern Uttar Pradesh, India. Methods: Multiple sequence alignment was done by MAFFT online multiple sequence alignment tools by selecting the necessary parameters. The phylogenetic study of ITS1 gene revealed all the three isolates (T. evansi horse India Uttar Pradesh- North Eastern Plane Zone, T. evansi mule India Uttar Pradesh- Eastern Plane Zone and T. evansi horse India Uttar Pradesh- Vindhyan Zone) of T. evansi were clustered in single group. Result: Multiple alignment of nucleotide sequence of ITS1 genes showed that T. evansi isolates from Eastern region of Uttar Pradesh, Indian had greater than 99% nucleotide homology with isolates of camel Iran (KX898420), camel Egypt (MW603779.1) and buffalo China (FJ712715.1).