Direct comparison of an ultrasensitive real-time PCR assay with droplet digital PCR for the detection of PIK3CA hotspot mutations in primary tumors, plasma cell-free DNA and paired CTC-derived gDNAs
This low-cost, high-throughput and ultrasensitive real-time PCR assay provides accurate and specific detection of <i>PIK3CA</i> hotspot mutations in liquid biopsy samples and gives similar results to ddPCR. This assay can be performed in labs where digital PCR instrumentation is not available. In CTC-derived gDNA and paired plasma-cfDNA, <i>PIK3CA</i> mutations detected were not identical, revealing that CTC and plasma-cfDNA give complementary information.
Authors
- Helena Linardou (ORCID: https://orcid.org/0000-0002-2906-6607)
- Evi Lianidou (ORCID: https://orcid.org/0000-0002-7796-5914)
- Alkistis Papatheodoridi (ORCID: https://orcid.org/0000-0001-6248-6964)
- Christos Papadimitriou (ORCID: https://orcid.org/0000-0001-8084-0445)
- Amanda Psyrri (ORCID: https://orcid.org/0000-0002-4321-766X)
- Stavroula Smilkou (ORCID: https://orcid.org/0000-0002-5338-0276)
- Athina Markou (ORCID: https://orcid.org/0000-0001-7760-9701)
- Evangelia D. Razis (ORCID: https://orcid.org/0000-0003-3873-2947)
- Loukas Kaklamanis
- Flora Zagouri
- Ioanna Balgouranidou
- Stylianos Kakolyris
Institutions
- National and Kapodistrian University of Athens (GR)
- Alexandra Hospital (GR)
- Onassis Cardiac Surgery Center (GR)
- Hygeia Hospital (GR)
- University General Hospital Attikon (GR)
- Metropolitan Hospital (GR)
- University Hospital of Alexandroupolis (GR)
Publication Details
- Journal
- Frontiers in Oncology
- Published
- 2024-12-06
- DOI
- https://doi.org/10.3389/fonc.2024.1435559
- Citations
- 2
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.57