Changes in N2O emissions from agricultural water samples with storage time at low temperature measured with headspace methods

This study aimed to evaluate the changes in nitrous oxide (N 2 O) emissions from agricultural water samples stored under different conditions, specifically refrigerated at 4C and frozen at -16C, over various time periods using the headspace method.Water samples were collected from agricultural runoff and analyzed for water nutrients parameters, including pH, EC, TN, TOC, PO 4 -P.N 2 O emissions were measured immediately after sample collection (0 day) and after storage for 5 and 7 days.The results indicated that N 2 O emissions decreased significantly over time, with a marked reduction in both refrigerated and frozen samples.In refrigerated samples, N 2 O emissions decreased by 41% after 5 days and by 78% after 7 days.In frozen samples, a similar trend was refrigerated samples, with emissions decreasing by 60% after 5 days and by 66% after 7 days.These findings highlight the critical impact of storage conditions and duration on the accuracy of N 2 O emission measurements.These findings can inform better practices for assessing indirect N 2 O emissions in agricultural water management.