Efficacy of Surgically Extracted Sperm in Assisted Reproductive Technology

Objectives The objective of this study is to evaluate and compare the efficacy of various sperm extraction techniques used in Assisted Reproductive Technology (ART), focusing on sperm retrieval rates, fertilization rates, and clinical outcomes in patients undergoing fertility treatments. Material and Methods This retrospective observational study was conducted at RISAA IVF, Green Park, New Delhi, from January 2023 to January 2024. The study included male participants diagnosed with azoospermia, who underwent ART procedures such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Sperm extraction techniques evaluated include percutaneous epididymal sperm aspiration (PESA), testicular sperm aspiration (TESA), and microdissection testicular sperm extraction (micro-TESE). Data were collected from electronic medical records, laboratory reports, and patient charts, focusing on demographic data, sperm retrieval rates, and clinical outcomes. Statistical analysis was performed using SPSS software, with significance set at P < 0.05. Results Surgical sperm retrieval was performed on 11 azoospermic patients. Viable sperm was successfully retrieved using PESA in 18.1% of the patients, while TESA and micro-TESE yielded successful retrievals in 63.6% and 18.1% of the cases, respectively. The overall pregnancy rate achieved through these procedures was 63.6%. No significant difference in pregnancy rates was observed between the different sperm retrieval techniques (P > 0.05). Conclusion The study highlights that PESA, TESA, and micro-TESE are effective sperm extraction techniques for azoospermic patients undergoing ART. The success in sperm retrieval and subsequent pregnancy outcomes underscores the importance of selecting the appropriate method based on individual patient conditions. These findings contribute to the evidence base for optimizing fertility treatments in patients with severe male factor infertility.