Protocol for chemogenetic activation of basal ganglia D1-MSNs and behavioral assessments in a primate Parkinson’s disease model
A circuit-based gene therapy strategy for Parkinson's disease (PD) has been shown to significantly reverse core symptoms in both murine and primate PD models. Here, we present a comprehensive workflow to specifically manipulate dopamine receptor D<sub>1</sub>-expressing medium spiny neurons by retrograde adeno-associated virus (AAV) transduction and chemogenetic activation using a designer toolkit. We describe steps for AAV injections and PD primate model induction. We then detail behavioral measurements to assess the therapeutic efficacy of the therapy for motor symptoms.
Authors
- Jianbang Lin (ORCID: https://orcid.org/0000-0001-5878-5806)
- Yuyuan Zhu
- Taian Liu (ORCID: https://orcid.org/0000-0002-8783-3165)
- Zhonghua Lu (ORCID: https://orcid.org/0000-0002-7102-5956)
- Ming Guo
- Zexuan Hong (ORCID: https://orcid.org/0000-0002-6467-3787)
- Yuantao Li (ORCID: https://orcid.org/0000-0002-0110-2717)
- Ting Yan (ORCID: https://orcid.org/0000-0003-1182-9079)
- Siyang Chen (ORCID: https://orcid.org/0000-0001-7471-4274)
- Shijing Feng (ORCID: https://orcid.org/0000-0001-5958-9161)
- Mengqi Li (ORCID: https://orcid.org/0000-0002-1171-5648)
- Ronghui Li (ORCID: https://orcid.org/0000-0001-6329-5895)
- Jianqing Zhang (ORCID: https://orcid.org/0000-0002-5822-2218)
- Yefei Chen (ORCID: https://orcid.org/0000-0001-5386-4863)
- Yi Yang (ORCID: https://orcid.org/0009-0009-7760-333X)
Institutions
- Chinese Academy of Sciences (CN)
- Shenzhen Maternity and Child Healthcare Hospital (CN)
- Shenzhen Institutes of Advanced Technology (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103470
- Citations
- 2
- Primary Topic
- Neurological disorders and treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.74
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- China Postdoctoral Science Foundation
- Science, Technology and Innovation Commission of Shenzhen Municipality