Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose
2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.
Authors
- Hsiao-Lin Lee (ORCID: https://orcid.org/0000-0002-4828-3383)
- Jeung‐Hee Lee (ORCID: https://orcid.org/0000-0002-4224-771X)
- Chin‐Feng Hsuan (ORCID: https://orcid.org/0000-0002-0562-8310)
- Daniel Liao
- Chih-Neng Hsu
- Ya‐Wen Liu (ORCID: https://orcid.org/0000-0003-0180-4142)
- Siow‐Wey Hee (ORCID: https://orcid.org/0000-0002-2055-2860)
- Karen Chia-Wen Liao (ORCID: https://orcid.org/0000-0001-6990-7928)
- Wei‐Shun Yang (ORCID: https://orcid.org/0000-0002-3249-280X)
- Tung-Yuan Lee (ORCID: https://orcid.org/0009-0001-2779-8361)
- Huaizhou Li
- Yi‐Cheng Chang (ORCID: https://orcid.org/0000-0002-8077-5011)
- Gwo‐Yu Chuang (ORCID: https://orcid.org/0000-0003-0375-1013)
- Tony Pan-Hou (ORCID: https://orcid.org/0009-0003-2185-8938)
- Chih‐Feng Wu (ORCID: https://orcid.org/0000-0001-5771-8062)
- Shao-Lun Chu
- Wenyi Li
Institutions
- National Taiwan University (TW)
- University of Chicago (US)
- Institute of Biological Chemistry, Academia Sinica (TW)
- National Taiwan University Hospital (TW)
- National Hsinchu University of Education (TW)
- National Yunlin University of Science and Technology (TW)
- I-Shou University (TW)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103478
- Primary Topic
- Cancer, Hypoxia, and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Council
- Academia Sinica
- National Taiwan University Hospital
- National Taiwan University
- National Taiwan University Hospital Yunlin Branch