Structural and molecular properties of mumps virus inclusion bodies

Viral RNA synthesis of mononegaviruses occurs in cytoplasmic membraneless organelles called inclusion bodies (IBs). Here, we report that IBs of mumps virus (MuV), which is the causative agent of mumps and belongs to the family <i>Paramyxoviridae</i>, displayed liquid organelle properties formed by liquid-liquid phase separation. Super-resolution microscopic analysis of MuV IBs demonstrated that nucleocapsid and phospho (P)-proteins formed a cage-like structure and that the viral polymerase adopted a reticular pattern and colocalized with viral RNAs. In addition, we characterized host RNAs localized in MuV IBs by a spatial transcriptome analysis, and found that RNAs containing G-quadruplex motif sequences (G4-RNAs) were concentrated. An in vitro phase separation assay showed that the G4-RNAs interacted with the P protein and enhanced condensation in P droplets. Together, our data show that MuV generates IBs with a characteristic cage-like structure and host G4-RNAs play an important role in forming MuV IBs.

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Publication Details

Journal
Science Advances
Published
2024-12-06
DOI
https://doi.org/10.1126/sciadv.adr0359
Citations
7
Primary Topic
RNA Research and Splicing
Type
article
Field-Weighted Citation Impact
1.64

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article

Structural and molecular properties of mumps virus inclusion bodies

Michiyo Kataoka, Yuki Kitai, Hirotaka Kobayashi, Tadaki Suzuki et al.
7 citations
Science Advances
RNA Research and Splicing
1.64
article

Structural and molecular properties of mumps virus inclusion bodies

Michiyo Kataoka, Yuki Kitai, Hirotaka Kobayashi, Tadaki Suzuki, Fumihiro Kato, Shinya Oki, Tsuyoshi Sekizuka, Yasuyuki Ohkawa, Makoto Takeda, Noriyo Nagata, Ken Matsuoka, Hiroshi Katoh, Ryuichi Kimura, Yukiko Akahori, Mika Hosogi
article en
7 citations

Abstract

Viral RNA synthesis of mononegaviruses occurs in cytoplasmic membraneless organelles called inclusion bodies (IBs). Here, we report that IBs of mumps virus (MuV), which is the causative agent of mumps and belongs to the family <i>Paramyxoviridae</i>, displayed liquid organelle properties formed by liquid-liquid phase separation. Super-resolution microscopic analysis of MuV IBs demonstrated that nucleocapsid and phospho (P)-proteins formed a cage-like structure and that the viral polymerase adopted a reticular pattern and colocalized with viral RNAs. In addition, we characterized host RNAs localized in MuV IBs by a spatial transcriptome analysis, and found that RNAs containing G-quadruplex motif sequences (G4-RNAs) were concentrated. An in vitro phase separation assay showed that the G4-RNAs interacted with the P protein and enhanced condensation in P droplets. Together, our data show that MuV generates IBs with a characteristic cage-like structure and host G4-RNAs play an important role in forming MuV IBs.

Science AdvancesVol. 10(49)
Kyushu University (JP), Kyoto University (JP), National Institute of Infectious Diseases (JP), The University of Tokyo (JP), Kumamoto University (JP)
University of Tokyo
Life in Land
Openalex Percentile: Top 16%
RNA Research and Splicing
1.64
DOIOpen Access PDFOpenAlex
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