Crystallographic analysis of G-clamp–RNA complex assisted by large scale RNA-binding profile
We present the X-ray crystal structure of a complex between a G-clamp and an internal loop motif of pre-mir-125a, selected from high affinity RNAs identified in a large-scale RNA-binding profile. This X-ray crystal structure reveals that the G-clamp interacts with three distinct guanine bases, forming robust bonds through hydrogen bonding and stacking interactions.
Authors
- Kaoru R. Komatsu (ORCID: https://orcid.org/0000-0002-4505-0840)
- Emi Miyashita
- Fumi Nagatsugi (ORCID: https://orcid.org/0000-0002-5526-6020)
- Kazumitsu Onizuka (ORCID: https://orcid.org/0000-0001-9713-4619)
- Ryosuke Nagasawa (ORCID: https://orcid.org/0009-0002-0474-3218)
- Kazunori Kawamura (ORCID: https://orcid.org/0000-0001-6786-678X)
- Hirohide Saito (ORCID: https://orcid.org/0000-0002-8570-5784)
- Jiro Kondo (ORCID: https://orcid.org/0000-0002-5682-3685)
- Hirotaka Murase (ORCID: https://orcid.org/0000-0001-5747-191X)
- Kosuke Tsuzuki
Institutions
- Tohoku University (JP)
- Kyoto University (JP)
- Sophia University (JP)
Publication Details
- Journal
- Chemical Communications
- Published
- 2024-12-06
- DOI
- https://doi.org/10.1039/d4cc04677c
- Citations
- 2
- Primary Topic
- RNA and protein synthesis mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Uehara Memorial Foundation
- Japan Society for the Promotion of Science
- Fusion Oriented REsearch for disruptive Science and Technology