Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome

N-glycome analysis of individual proteins and tissues is crucial for fundamental and applied biomedical research and medical diagnosis and plays an important role in the evaluation of the quality of biopharmaceutical and biotechnological products. The interest in this research area continues to grow annually, thereby increasing the demand for the high-throughput profiling of human blood plasma N-glycome. In response to this need, we have developed an optimized, simple, and rapid protocol for the N-glycome profiling of human plasma proteins. This protocol encompasses the entire analysis cycle, from plasma isolation to N-glycan spectrum quantification. While the proposed method may have lower efficiency compared to already published high-throughput methods, its adaptability makes it suitable for implementation in virtually any molecular biological laboratory.

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Publication Details

Journal
Biomolecules
Published
2024-12-04
DOI
https://doi.org/10.3390/biom14121551
Citations
1
Primary Topic
Glycosylation and Glycoproteins Research
Type
article
Field-Weighted Citation Impact
0.23

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Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome

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Glycosylation and Glycoproteins Research
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article

Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome

Yurii S. Aulchenko, Anna Timoshchuk, Maja Hanić, Anna G. Soplenkova, Д. Е. Маслов, Т. С. Фролова, Sodbo Sharapov, O. N. Leonova, Mikhail V. Golubev, Alexander A. Bondar
article en
1 citations

Abstract

N-glycome analysis of individual proteins and tissues is crucial for fundamental and applied biomedical research and medical diagnosis and plays an important role in the evaluation of the quality of biopharmaceutical and biotechnological products. The interest in this research area continues to grow annually, thereby increasing the demand for the high-throughput profiling of human blood plasma N-glycome. In response to this need, we have developed an optimized, simple, and rapid protocol for the N-glycome profiling of human plasma proteins. This protocol encompasses the entire analysis cycle, from plasma isolation to N-glycan spectrum quantification. While the proposed method may have lower efficiency compared to already published high-throughput methods, its adaptability makes it suitable for implementation in virtually any molecular biological laboratory.

BiomoleculesVol. 14(12)
Immanuel Kant Baltic Federal University (RU), Lomonosov Moscow State University (RU), Institute of Chemical Biology and Fundamental Medicine (RU), Central Scientific Research Institute of Traumatology and Orthopedics (RU), Institute of Cytology and Genetics (RU), Genos (Croatia) (HR), Siberian Branch of the Russian Academy of Sciences (RU), Science Research Laboratory (US)
Ministry of Science and Higher Education of the Russian Federation
Industry, innovation and infrastructure
Openalex Percentile: Top 40%
Glycosylation and Glycoproteins Research
0.23
DOIOpen Access PDFOpenAlex
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