Molecular and metabolomic characterization of hiPSC-derived cardiac fibroblasts transitioning to myofibroblasts
Our study highlights that the hiPSC-CF passaging enhanced fibroblast activation, activated fibrotic signaling pathways, and enhanced mitochondrial metabolism approximating what has been reported in primary cardiac fibroblasts. Thus, hiPSC-CFs may provide an accurate <i>in vitro</i> preclinical model for the cardiac fibrotic condition, which may facilitate the identification of putative anti-fibrotic therapies, including patient-specific approaches.
Authors
- Robert A. Rose (ORCID: https://orcid.org/0000-0001-9880-3482)
- Raghu S. Nagalingam (ORCID: https://orcid.org/0000-0003-1095-8303)
- Michael P. Czubryt (ORCID: https://orcid.org/0000-0003-4897-9804)
- Glen F. Tibbits (ORCID: https://orcid.org/0000-0002-2586-3115)
- Philipp F. Lange (ORCID: https://orcid.org/0000-0003-1171-5864)
- Ian Dixon (ORCID: https://orcid.org/0000-0002-3763-5961)
- Saif Dababneh (ORCID: https://orcid.org/0000-0003-1593-0756)
- Ramon I. Klein Geltink
- Homa Hamledari
- Farah Jayousi
- Dina Hosseini
- Chloe Lindsay
Institutions
- University of British Columbia (CA)
- University of Calgary (CA)
- Simon Fraser University (CA)
- St. Boniface Hospital (CA)
- BC Children's Hospital (CA)
- University of Manitoba (CA)
- Libin Cardiovascular Institute of Alberta (CA)
Publication Details
- Journal
- Frontiers in Cell and Developmental Biology
- Published
- 2024-12-04
- DOI
- https://doi.org/10.3389/fcell.2024.1496884
- Citations
- 3
- Primary Topic
- Cardiac Fibrosis and Remodeling
- Type
- article
- Field-Weighted Citation Impact
- 1.21
Funders
- Heart and Stroke Foundation of Canada
- Children's Hospital Foundation
- BC Children's Hospital
- Stem Cell Network
- Michael Smith Health Research BC
- Canadian Institutes of Health Research