Greenness assessment approach for spectrofluorimetric detection of daptomycin using metal oxide nanosensors synthesized via Commiphora myrrha extract in the presence of organizing solution

• Greenness evaluation for daptomycin spectrofluorimetric detection. • Synthesis of metal oxide nanosensors from Commiphora Myrrha . • Characterization of nanoparticles composed of zinc oxide. • Validation of the proposed methodology. • Implementation of the recommended methodology. Daptomycin (DPT) is a lipopeptide antibiotic used primarily to treat severe infections caused by Gram-positive bacteria. A spectrofluorimetric detection of DPT using metal oxide nanosensors synthesized via Commiphora myrrha extract in the presence of an organizing solution is presented in this study. The study describes a unique analytical method for detecting daptomycin (DPT) in pharmaceutical formulations, which employs a novel fluorescent system based on zinc oxide nanoparticles (ZnO-NPs) and cetylpyridinium chloride (CPC). The ZnO-NPs were produced using an environmentally friendly technique that included Commiphora myrrha extract, which serves as both a reducing and stabilizing agent in the green synthesis process. The morphology, size, and structural properties of the ZnO-NPs were carefully investigated utilizing a variety of spectroscopic and microscopic methods. The new method makes use of the unique fluorescence capabilities of ZnO-NPs in the presence of CPC for the highly sensitive and selective detection of DPT. This method facilitates precise quantification over a wide calibration range of 0.05–10 µg/mL, exhibiting remarkable sensitivity. The method is highly accurate, with DPT recoveries typically surpassing 99 %, indicating its reliability. Intermediate precision was utilized to evaluate both accuracy and precision in accordance with methodological requirements. The greenness of the method assessment emphasizes its ecologically friendly approach, which uses Commiphora myrrha extract for the sustainable synthesis of ZnO-NPs, minimizing environmental effects while maintaining strong analytical performance.