Effects of optimized interaction of short-term hypergravity stimulation and nitrate-deficient cultivation in maize root using genetic-immunological algorithms

• Hypergravity boosts root-to-shoot ratio and vegetative growth of maize seedlings in low-nitrate hydroponics • Integrated MGGP-AIS algorithms optimize R/S = f {2 g time exposure}, improved productivity yields • Conductive tissues of maize adapted in nutrient-stressed environment after exposure to hypergravity • Hypergravity stimulation of corn seeds can reduce the introduction of nutrient fertilizers in the long run Nitrate is a macronutrient substantial for plant root and shoot growth, however, the availability of nitrate within soil-based and soilless cultivation environments is not consistently optimal, presenting a significant challenge for plant growth and development. Traditional seed stimulation includes scarification, soaking, hormone application and microbial application but they are all invasive. This study pioneered an experimental approach to address the challenges posed by nutrient deficiency in hydroponic environment by integrating Multigene Genetic Programming (MGGP) with immunological computation algorithms, namely Clonal Selection Algorithm (CSA), Ant Colony Optimization Algorithm (ACOA), and COVID Optimization Algorithm (COVIDOA) in determining the exact optimal time exposure to 2 g hypergravity that can induced the growth of three maize genotypes (PSB 92-97, NSIC CN 302, and NSIC CN 282). Through varying dry seed exposure times to hypergravity (6, 12, and 24 h), labeled models gCSA, gACOA, and gCOVIDOA converged to 20.120 h, 22.466, and 19.700 h, respectively, based on the formulated 2-gene model of root-to-shoot ratio as a function of exposure time. Exposure time between 20-24 h increased the root-to-shoot ratio (R/S) by at least a factor of 2.631 and the seedling's dry weight by 13.430 g while between 10-15 h of exposure reduced the overall biomass. gACOA-treated seedings exhibited an R/S of 3.732 ± 0.067 having the highest uniformity among the control, gCSA, and gCOVIDOA treatments. gACOA-treated seedlings have healthier root hair compared to unexposed seeds after 14 days and revealed the highest rate of increase in metaxylem, xylem, phloem, and radicle diameters with a factor of 3.651 μm/hr, 1.440 μm/hr, 0.872 μm/hr, and 71.602 μm/hr of exposure in 2 g hypergravity. This study implies that stimulating corn seeds using hypergravity can help lessen the introduction of nutrient fertilizers in the long run which could help in reducing the farm expenses.