Thermobifida fusca Cel6B moves bidirectionally while processively degrading cellulose
The unexpected motility patterns of TfCel6B are likely due to previously unknown mechanisms of processive cellulase motility implicating irregularities in cellulose substrate ultrastructure. While TfCel6B is processive, it has low motility at room temperature. Factors that most dramatically impact enzyme velocity are temperature and the presence of its native carbohydrate-binding module and linker. In contrast, substrate ultrastructure and applied force did not greatly impact velocity. These findings motivate further study of TfCel6B for its engineering and potential implementation in industrial processes.
Authors
- Bhargava Nemmaru (ORCID: https://orcid.org/0000-0002-5005-4678)
- Shishir P. S. Chundawat (ORCID: https://orcid.org/0000-0003-3677-6735)
- Madeline M. Johnson (ORCID: https://orcid.org/0000-0002-9518-293X)
- Matthew J. Lang
- Antonio DeChellis (ORCID: https://orcid.org/0000-0002-0181-4984)
Institutions
- Rutgers, The State University of New Jersey (US)
- Vanderbilt University (US)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2024-12-04
- DOI
- https://doi.org/10.1186/s13068-024-02588-0
- Citations
- 3
- Primary Topic
- Biofuel production and bioconversion
- Type
- article
- Field-Weighted Citation Impact
- 0.55
Funders
- National Science Foundation
- U.S. Department of Energy
- National Institutes of Health
- Office of Science
- National Institute of General Medical Sciences
- Division of Chemical, Bioengineering, Environmental, and Transport Systems