Low-cost optical fiber multimode interference biosensor based on a glucose sensitive Glucose-Oxidase enzyme thin-film

• Low-cost optical fiber biosensors applied for glucose monitoring. • SMS biosensors based on Glucose-Oxidase enzyme thin-film fabricated by LbL. • Glucose characterization in a clinical range from 0.3 to 2.4 mg mL-1. • Response time of 9 seconds and a sensitivity of 1.8 nm/(mg mL-1) • Capability to detect hypoglycemia and diabetes glucose ranges. In this research we report a contribution for the development of low-cost fiber optical biosensors fabricated by the Single Mode-Multi Mode-Single Mode configuration applied for the glucose monitoring considering clinical concentrations ranges in aqueous analytes. Designed devices are evaluated using health standard detection ranges, such as healthy, pre-diabetic, and diabetic stages operating at the visible spectral region. The sensing regions has been prepared by the etching technique in order to improve the interaction between the evanescent wave with the surrounding medium followed by functionalization of enzyme oxidase glucose via the electrostatic self-assembly using by Poly(allylamine hydrochloride) as an immobilizer matrix. The increase of bilayers number over the sensor surface permits us to demonstrate the enhancement of sensitivity and limit of detection. Experimental results permitted the glucose characterization in the range from 0.3 to 2.4 mg mL-1 obtaining a response time of 9 seconds and a sensitivity of 1.8 nm/(mg mL-1) allowing to detect hypoglycemia and diabetes stages according to the World Health Organization standards.