Results of the 2023 rapid DNA multi-laboratory study – I-Chip

Identical sensitivity and mixture sample sets were provided to six external laboratories to evaluate the recent enhancements to the Rapid DNA technology for crime scene-type samples. Data were returned to the Federal Bureau of Investigation and the National Institute of Standards and Technology for review and analysis. Manual interpretation (modified Rapid DNA analysis) was conducted on all samples to assess the success of genotyping for both full profiles and the 20 Combined DNA Index System (CODIS) core STR loci using 18 total cellular DNA input amounts ranging from 102.4 ng to 0.025 ng. Two-person and three-person mixtures at varying ratios for two total DNA inputs were analyzed and observed allele percentages were compared to expected allele percentages. • Assessment of the enhanced I-Chip across seven ANDE 6 C instruments • Consistency within the heatmap demonstrates there are little to no sensitivity differences between instruments • Full profiles were obtained down to 6.4 ng of total cellular DNA in blood samples