A rolling circle amplification-based DNAzyme walker against intracellular degradation for imaging tumor cells' microRNA
We present a novel DNA molecular machine (RCA-D-Walker) that integrates a DNAzyme-based molecular beacon with RCA-based vectors for miRNA imaging in tumor cells. It can accurately target tumor cells through the sgc8 aptamer. The target miRNA can restore the DNAzyme's ability to cleave the substrate, which in turn produces an amplified fluorescent signal. The RCA-D-Walker exhibits enhanced tumor cell targeting, improved cell permeability, and greater resistance to nuclease degradation. Utilizing this strategy, we achieved accurate and efficient imaging of miRNA-21 in tumor cells.
Authors
- Pengfei Shi (ORCID: https://orcid.org/0000-0002-9657-1478)
- Huimin Niu (ORCID: https://orcid.org/0000-0002-4270-6437)
- Haoqi Yang (ORCID: https://orcid.org/0000-0001-6706-8470)
- Shujuan Sun (ORCID: https://orcid.org/0000-0002-2753-7698)
- Shusheng Zhang (ORCID: https://orcid.org/0000-0002-1006-9728)
- Chenxiao Zhao
Institutions
- Fujian Medical University (CN)
- Linyi University (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2024-12-04
- DOI
- https://doi.org/10.1039/d4cc05440g
- Citations
- 4
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Fujian Province
- Linyi University
- Natural Science Foundation of Shandong Province
- Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China