Evaluating the Impact of Rapid Thermoresistance (RTT) on Membrane and Chromatin Features in Post-Thawed Bovine Semen

This work aims to evaluate the effects of rapid thermoresistance on the morphofunctional aspects of membranes using flow cytometry, as well as the quality of sperm chromatin using the Toluidine Blue technique, both before and after the thermoresistance test.Six sets of frozen semen from ten bulls were used. The samples were thawed in a water bath at 36ºC and separated into two aliquots. The first aliquot, control group (C), was kept in a test tube previously heated to 36ºC with subsequent evaluations using microscopy, cytometry, and chromatin analyses. The second aliquot, experimental group (E), underwent the rapid thermoresistance test (RTT) in a water bath preheated at 46ºC for 30 minutes with subsequent evaluations using microscopy, cytometry, and chromatin analyses. For statistical analysis, the paired T test, significance (P<0.05), was used.