Neuroprotective Effect of Naturally Isolated Scopoletin (ISCN) from 3 Selected Ipomoea Species on Learning and Memory Impairment in Amnestic Mice Model

Background: The Convolvulceae family, which includes the several climbing herbs and weeds in the genus Ipomoea, is widely used in Ayurvedic, Unani and Siddha medicine. These plants are found across India. The current study to explore the Neuroprotective effect of naturally isolated scopoletin (ISCN) compound from Ipomoea reniformis (Choisy), Ipomoea cairica (L.) and Ipomoea triloba (L.) on learning and memory impairment in amnestic Swiss albino mice model. Methods: There was no mortality observed at intraperitoneal maximum dosages up to 100 mg/kg body weight; the ISCN showed a significant safety margin. The Neuroprotective Effect of ISCN was evaluated by taking various doses (3, 6, 12 mg/kg i.p), standard piracetam (150 mg/kg; i.p) and scopolamine (1 mg/kg; i.p.)to produce amnesia in mice by using Interoceptive includes estimation of acetyl cholinesterase (AchE) activity and extroceptive animal models include the Morris-water maze (MWM), object recognition test (ORT), passive avoidance test (PAT). Result: The memory score significantly improved after dosing of ISCN and standard piracetam in comparison with scopolamine-producing amnesia in mice. Dose-dependent ISCN shows significant (P less than 0.01) improvement in Escape Latency in MWM, improvement in step-down latency in PAT, mice spent more time exploring the novel object in ORT and dose-dependent ISCN shows significant inhibition of AchE in mice brain. This finding indicates that ISCN may exert a neuroprotective effect by inhibiting the AchE enzyme. ISCN has the potential to be therapeutically beneficial for reducing some of the memory impairments linked to Alzheimer’s.