The Investigation of the Effect of Boron on Intestinal Incision Wound Healing in Rats

In this study 250-300 g 38 adult male Wistar Albino rats were used, randomly divided into six groups as four groups that received boron (n=7/group) and two control groups (n=5/group). Etibor-48 (Borax pentahydrate) was diluted in saline and administered by gavage. The Boron 1 group received 10 mg/kg boron for three days and the Boron 2 group received 10 mg/kg for seven days, Boron 3 group received 30 mg/kg for three days, and the Boron 4 group, received 30 mg/kg for seven days before the start of the study. On day 0, colon incisional wound was performed for all rats under general anesthesia and sutured. Control groups took no drugs and were euthanized on the third and seventh postoperative days. Blood sampling were done via cardiac punction at necropsy to measure Interleukin-1, IL-6, TNF-α, MPO, MDA, NO, GSH, and AOA. The sutured bowel incision line was evaluated histopathologically. Inflammatory cell measurement results in Boron 1, 2, 3, and 4 groups, and Control 3- and 7-day groups were 2.93±0.41, 3.93±0.41, 3.61±0.55, 3.93±0.41, 1.26±0.41, and 1.43±0.51, respectively. There were statistical difference between the groups (P˂0.05). AOA measurement results in the Boron 1, 2, 3, and 4 groups, and Control 3- and 7-day groups were 7.38±0.64, 8.27±0.57, 9.07±1.16, 9.06±0.86, 10.00±1.47, and 9.86±0.54 mmol/L, respectively. There were statistical difference between the Boron 1 and the Control 3- and 7-day groups (P˂0.05). It is concluded that Borax pentahydrate solution 30 mg/kg had a positive effect on intestinal incisional wound healing, contrary to the literature.