Protocol to culture Leishmania protozoans for lipophosphoglycan extraction and purification
Lipophosphoglycan (LPG) is a macromolecule on the surface of Leishmania spp. parasites. The biochemical structure of LPG varies throughout the parasites' life cycle between proliferative (procyclic) and infective (metacyclic) stages, as well as between species and strains. Here, we outline a protocol for growing Leishmania parasites in vitro to harvest LPG. We describe steps for parasite differentiation and LPG extraction and purification. LPG has applications in medical research, such as in trained immunity and immunotherapy for cancer. For complete details on the use and execution of this protocol, please refer to dos Santos et al.<sup>1</sup>.
Authors
- Jéssica Cristina dos Santos (ORCID: https://orcid.org/0000-0001-9191-7356)
- Lisa U. Teufel (ORCID: https://orcid.org/0000-0003-4287-5908)
Institutions
- Radboud University Nijmegen (NL)
- Radboud University Medical Center (NL)
- Radboud Institute for Molecular Life Sciences (NL)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103468
- Citations
- 1
- Primary Topic
- Research on Leishmaniasis Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.54
Funders
- East Tennessee State University
- Radboud Universiteit
- Radboud Universitair Medisch Centrum