Mucosal SARS-CoV-2 S1 adenovirus-based vaccine elicits robust systemic and mucosal immunity and protects against disease in animals
This publication presents an assessment of the immune response and effectiveness of a vaccine containing genetically modified non-replicating recombinant that expresses the S1 subunit protein of SARS-CoV-2. We conducted a comparative analysis of the immune response potency, durability, and protective effectiveness of this vaccine using intramuscular (IM) and intranasal (IN) inoculation in mice and Syrian hamsters. Our findings indicate that both vaccinations were effective in stimulating strong and long-lasting immune responses, both locally and across the body, when administered through either IM or IN methods. Crucially, our study demonstrated that the IN vaccination outperformed the IM vaccine by effectively and significantly suppressing the multiplication of the virus in the lungs and nasal turbinates. Additionally, the IN vaccine provided protection against disease-related weight loss and lung damage in the animals. This work showcases the potential of intranasal administration as a viable method to stimulate both mucosal and systemic immunity. This technique provides improved defense against SARS-CoV-2 and maybe additional variations.
Authors
- Abdullah Algaissi
- Reem M. Alsulaiman
- Asalah Helal
- Levi A. Tamming (ORCID: https://orcid.org/0000-0003-2851-7311)
- Jegarubee Bavananthasivam
- Anwar M. Hashem (ORCID: https://orcid.org/0000-0002-8471-7011)
- Rwaa H. Abdulal
- Mohammad Basabrain (ORCID: https://orcid.org/0000-0003-1925-0424)
- Najwa D. Aljehani
- Rowa Y. Alhabbab (ORCID: https://orcid.org/0000-0001-7135-9967)
- Anh Tran (ORCID: https://orcid.org/0000-0003-0644-6014)
- Khalid A. Alluhaybi
- Aishah Ghazwani (ORCID: https://orcid.org/0000-0001-7576-8418)
- Tarfa Altorki (ORCID: https://orcid.org/0009-0001-0374-2187)
- Salima Shebbo
- Rahaf Alharbi (ORCID: https://orcid.org/0000-0002-9119-8360)
- Wangxue Chen
- Simon Sauvé (ORCID: https://orcid.org/0000-0002-9620-4889)
- Ahmad Bakur Mahmoud (ORCID: https://orcid.org/0000-0002-1515-2352)
- Diana Duque
- Turki S. Abujamel
- Wesam H. Abdulaal (ORCID: https://orcid.org/0000-0002-2510-1946)
- Mohammad A. Sanki
- M‐Zaki ElAssouli
- Mohamed A. Alfaleh (ORCID: https://orcid.org/0000-0003-3716-0019)
- Xuguang Li (ORCID: https://orcid.org/0000-0003-1155-9128)
- Muhammad Yasir Khan (ORCID: https://orcid.org/0009-0007-6372-9949)
- Farah Ayman Sukareh
- Faris H. Alyami
- Lisheng Wang (ORCID: https://orcid.org/0009-0003-6836-6156)
Institutions
- Health Canada (CA)
- University of Ottawa (CA)
- King Abdulaziz University (SA)
- Taibah University (SA)
- National Research Council Canada (CA)
- Qatar University (QA)
- Jazan University (SA)
Publication Details
- Journal
- mBio
- Published
- 2024-12-04
- DOI
- https://doi.org/10.1128/mbio.02170-24
- Citations
- 5
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 1.95
Funders
- King Abdulaziz University