Amorphous Magnesium Coating for Achieving Functional Changes from Antibacterial to Osteogenic Activities

Medical devices composed of titanium (Ti) should exhibit antibacterial and osteogenic activities to achieve both infection prevention and rapid bone reconstruction. Here, a Ti surface was modified by performing magnetron sputtering (MS) using pure Mg or Mg-30Ca alloy targets for surface functionalization. MC0, prepared with a pure Mg target, had a crystalline metallic-Mg coating layer, whereas MC30, prepared with an Mg-30Ca alloy target, had an amorphous coating composed of Mg and Ca. Both samples rapidly dissolved when immersed in a cell culture medium and exhibited antibacterial activities against methicillin-resistant <i>Staphylococcus aureus</i> and cytotoxicity against MC3T3-E1 cells. Furthermore, MC30 promoted the proliferation and calcification of MC3T3-E1 cells because of the subsequent deposition of calcite on the surface after rapid dissolution. Our findings are the first to reveal that MS performed by using an Mg-30Ca alloy target endowed Ti surfaces with functional changes from antibacterial to osteogenic activities over time. Our results provide fundamental insights into the surface design of Ti-based medical devices for enhanced bone reconstruction and infection prevention and offer possibilities for biomedical applications of Mg-based coatings.