Affinity purification of the outer hair cell motor protein prestin using His-tag
In the present study, 78.7 μg of prestin was purified from 2.0 × 10<sup>9</sup> CHO cells, which stably expressing 6 × His-tagged prestin, by extracting cell membrane fractions and standard affinity chromatography for His-tag.
Authors
- Michio MURAKOSHI (ORCID: https://orcid.org/0000-0002-2389-7995)
- Hisashi Sugimoto (ORCID: https://orcid.org/0000-0001-8903-4423)
- Tomokazu Yoshizaki (ORCID: https://orcid.org/0000-0002-2420-5540)
- Manabu Inaba
Institutions
- Kanazawa University (JP)
Publication Details
- Journal
- Auris Nasus Larynx
- Published
- 2024-12-03
- DOI
- https://doi.org/10.1016/j.anl.2024.11.002
- Primary Topic
- Hearing, Cochlea, Tinnitus, Genetics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Shibuya Science Culture and Sports Foundation
- Japan Society for the Promotion of Science