Antitumor potentials of onco-microbial in Chinese patients with pancreatic cancer

Recent studies have revealed that intratumoral microbiota is implicated in pancreatic cancer (PC), yet the spectra of intratumoral microbiota and their relationship with PC in Chinese patients remained to be clarified. In this study, tumor and paired paracancerous tissue from 53 patients were profiled by bacterial 16S rRNA gene sequencing. Both <i>α-</i> and <i>β</i>-diversity displayed significant differences between tumors and adjacent tissues, with higher diversity in tumors. Three bacteria phyla (Proteobacteria, Firmicutes, and Actinobacteria) were prevalent in both cancers and adjacent normal tissues. A high prevalence of <i>Pseudomonas</i> has been identified in the PC tumor microenvironment and was associated with prolonged overall survival. Furthermore, the results of <i>in vitro</i> experiments suggested that <i>Pseudomonas fluorescens</i> (<i>P. fluorescens</i>) could inhibit the proliferation and induce apoptosis of pancreatic cancer cells. These findings revealed distinctive microbial features of the PC tumors and normal tissues in Chinese populations and exhibited the antitumor potential of <i>P. fluorescens</i> in PC.