Comparative Evaluation of Antimicrobial Effect of Different Irrigating Solution Against Enterococcus Faecalis - An In-Vitro Study

BACKGROUND The invasion of microorganisms into the root canal system poses a significant challenge in endodontic infections, especially in primary teeth. Enterococcus faecalis is a common culprit in such infections, necessitating effective antimicrobial strategies. This study aims to assess various irrigating solutions' antimicrobial efficacy against E. faecalis. Aim: The aim of this study was to evaluate the antimicrobial efficacy of different irrigating solutions against E. faecalis. METHODS Six experimental groups, including lemon extract, chlorine dioxide solution, apple cider vinegar, Echinacea angustifolia, sodium hypochlorite, and a control group (normal saline), were evaluated. The antimicrobial activity was assessed using the agar-well diffusion method, and MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were determined. RESULTS Lemon extract and apple cider vinegar exhibited higher zones of inhibition compared to sodium hypochlorite, chlorine dioxide, and Echinacea angustifolia. Statistical analysis confirmed significant differences in antimicrobial efficacy between various groups. Lemon extract and apple cider vinegar demonstrated the lowest MIC and MBC values, indicating potent antimicrobial activity against E. faecalis. CONCLUSIONS This study highlights the antimicrobial efficacy of lemon extract and apple cider vinegar against E. faecalis, suggesting their potential as alternative irrigating solutions in endodontic practice. Further research is warranted to explore their clinical applicability and long-term effects on treatment outcomes.