A chemigenetic indicator based on a synthetic chelator and a green fluorescent protein for imaging of intracellular sodium ions
A chemigenetic indicator with an affinity suitable for imaging of intracellular sodium ions (Na<sup>+</sup>) in mammalian cells was developed. The indicator, based on a chimera of green fluorescent protein (GFP) and HaloTag labeled with a synthetic crown ether chelator, was produced by a combination of rational design and directed evolution. In mammalian cells the indicator exhibited an approximately 100% increase in excitation ratio when the cells were treated with 20 mM Na<sup>+</sup> and an ionophore.
Authors
- Takuya Terai (ORCID: https://orcid.org/0000-0002-3425-3589)
- Shiori Takeuchi (ORCID: https://orcid.org/0000-0003-2126-047X)
- Robert E. Campbell (ORCID: https://orcid.org/0000-0003-0604-092X)
- Shosei Imai (ORCID: https://orcid.org/0000-0003-0388-4764)
Institutions
- Université Laval (CA)
- The University of Tokyo (JP)
Publication Details
- Journal
- RSC Chemical Biology
- Published
- 2024-12-03
- DOI
- https://doi.org/10.1039/d4cb00256c
- Citations
- 1
- Primary Topic
- Molecular Sensors and Ion Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.24
Funders
- Konica Minolta Imaging Science Foundation
- Asahi Glass Foundation
- University of Tokyo
- Japan Society for the Promotion of Science