HPTLC detection, extraction - isolation optimization and characterization of in-house isolated Wedelolactone from the whole plant extract of <i>Eclipta prostrata</i> (L.) L. for the development of efficacious formulations.
In present study Wedelolactone (WL) was identified from successive extracts of whole plant <i>Eclipta prostrata</i> (L.) L. using HPTLC, optimised extraction/isolation method and confirmed WL structure.Extraction, isolation andidentification was performed though cold-percolation method, column chromatography and TLC respectively.WL was found abundantly in ethyl acetate and ethanol extracts at UV254 & 366 nm, respectively at R<i><sub>F</sub></i>0.42 by using toluene:ethyl acetate: formic acid (6:3.5:0.5, v/v/v). In the Method IV got a pure pale green crystals (0.138 g, % yield of 0.23) was adopted. Isolated compound verified by TLC and the structure confirmed by various spectroscopic tools. The study proposed high efficiency extraction-isolation method for WL, which could enhance the efficacy for identification of <i>E. prostrata,</i> studying such coumestan in extracts and herbal formulations. Study potentially aiding in developing advance anti-cancer and hepatoprotectivedosages and quality control.
Authors
- Murugammal Shanmugam
- Narayanam Srikanth (ORCID: https://orcid.org/0000-0002-3976-4978)
- Ch. Venkata Narasimhaji
- D. Velvizhi
- Rabinarayan Acharya (ORCID: https://orcid.org/0000-0003-4413-1937)
- Joginder Singh (ORCID: https://orcid.org/0000-0001-6968-4912)
- Arjun Singh (ORCID: https://orcid.org/0000-0002-4422-8694)
- Himanshu Sharma (ORCID: https://orcid.org/0000-0001-5741-2434)
Institutions
- Central Council for Research in Ayurvedic Science (IN)
- Ministry of AYUSH (IN)
Publication Details
- Journal
- Natural Product Research
- Published
- 2024-12-03
- DOI
- https://doi.org/10.1080/14786419.2024.2431128
- Primary Topic
- Medicinal Plants and Neuroprotection
- Type
- article
- Field-Weighted Citation Impact
- 0.00