Sanguibacter biliveldensis sp. nov., a Gram-positive mesophilic bacterium isolated from plant lesions

A bacterial strain was isolated from pathogenic lesions of <i>Acer campestre</i> tree leaves from the Teutoburg Forest in North Rhine-Westphalia, Germany, by culture on non-selective agar plates. 16S rRNA sequencing revealed 100% similarity to <i>Sanguibacter keddieii</i> and <i>Sanguibacter inulinus</i>, as well as 99% similarity to <i>Sanguibacter gelidistatuariae</i> and <i>Sanguibacter antarcticus</i>. Here, we used genome-based taxonomy with the Type (Strain) Genome Server (TYGS), which suggests the isolation of a novel prokaryotic strain. According to TYGS-analysis, using whole genome digital DNA-DNA hybridization, only 65.5% similarity to the closest relative <i>S. inulinus</i> was revealed, suggesting a novel species. Growth was observed at both aerobic and anaerobic conditions. Bacterial cells depicted coryneform motile rods, with a length of 1.1-3.3 µm and a constant diameter of 0.5 µm. Cells did not form spores under the tested conditions and stain Gram-positive. Growth occurred between 0.5 and 4% NaCl (optimal: 1%), at pH 5.5-9.5 (optimal: 8.0-9.0). The strain was mesophilic with an optimal growth at 25°C. Major cellular fatty acids of the novel strain were anteiso-C_{15 : 0} and C_{16 : 0}.