Derivation of two induced pluripotent stem cell lines from a healthy control subject
Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.
Authors
- Khine Zaw (ORCID: https://orcid.org/0000-0002-4121-1985)
- Leon M. Larcher (ORCID: https://orcid.org/0000-0001-8481-0083)
- Terri L. McLaren (ORCID: https://orcid.org/0000-0003-3195-669X)
- Fred K. Chen (ORCID: https://orcid.org/0000-0003-2809-9930)
- Luke Jennings (ORCID: https://orcid.org/0000-0001-8163-7158)
- Shang‐Chih Chen (ORCID: https://orcid.org/0000-0002-5746-6982)
- Tina M. Lamey (ORCID: https://orcid.org/0000-0002-4608-4073)
- Jennifer A. Thompson (ORCID: https://orcid.org/0000-0003-3553-6457)
- Di Huang (ORCID: https://orcid.org/0000-0001-5657-0625)
- Samuel McLenachan (ORCID: https://orcid.org/0000-0001-5732-7387)
- Dan Zhang (ORCID: https://orcid.org/0000-0003-1728-9069)
Institutions
- Lions Eye Institute (AU)
- Sir Charles Gairdner Hospital (AU)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2024-12-03
- DOI
- https://doi.org/10.1016/j.scr.2024.103621
- Citations
- 1
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- Channel 7 Telethon Trust
- Retina Australia
- McCusker Charitable Foundation
- National Health and Medical Research Council