Establishing a comprehensive web‐based analysis platform for <i>Nicotiana benthamiana</i> genome and transcriptome
Nicotiana benthamiana has long served as a crucial plant material extensively used in plant physiology research, particularly in the field of plant pathology, because of its high susceptibility to plant viruses. Additionally, it serves as a production platform to test vaccines and other valuable substances. Among its approximately 3.1 Gb genome, 57 583 genes have been annotated within a 61 Mb region. We created a comprehensive and easy-to-use platform to use transcriptomes for modern annotation. These tools allow to visualize gene expression profiles, draw molecular evolutionary phylogenetic trees of gene families, perform functional enrichment analyses, and facilitate output downloads. To demonstrate their utility, we analyzed the gene expression profiles of enzymes within the nicotine biosynthesis pathway, a secondary metabolic pathway characteristic of the Nicotiana genus. Using the developed tool, expression profiles of the nicotine biosynthesis pathway genes were generated. The expression patterns of eight gene groups in the pathway were strongly expressed in the roots and weakly expressed in leaves and flowers of N. benthamiana. The results were consistent with the established gene expression profiles in Nicotiana tabacum and provided insights into gene family composition and expression trends. The compilation of this database tool can facilitate genetic analysis of N. benthamiana in the future.
Authors
- Yasunori Ichihashi (ORCID: https://orcid.org/0000-0003-1935-4397)
- Hideki Hirakawa (ORCID: https://orcid.org/0000-0002-7128-7390)
- Jiyuan An (ORCID: https://orcid.org/0000-0002-8183-8248)
- Takamasa Suzuki (ORCID: https://orcid.org/0000-0002-1977-0510)
- Christopher Winefield (ORCID: https://orcid.org/0000-0002-6262-6370)
- Sachiko Isobe (ORCID: https://orcid.org/0000-0002-9555-5054)
- Kenta Shirasawa (ORCID: https://orcid.org/0000-0001-7880-6221)
- Peter M. Waterhouse (ORCID: https://orcid.org/0000-0002-2812-3753)
- Kenji Miura (ORCID: https://orcid.org/0000-0003-1262-2176)
- Yasuhiro Tanizawa (ORCID: https://orcid.org/0000-0002-6294-3309)
- Yasukazu Nakamura (ORCID: https://orcid.org/0000-0002-6782-5715)
- Michitaka Notaguchi (ORCID: https://orcid.org/0000-0003-0831-2108)
- Ken‐ichi Kurotani (ORCID: https://orcid.org/0000-0003-1618-8557)
- Koya Tagiri
- Moe Mori
- Abedelaziz Ramadan
Institutions
- National Institute of Genetics (JP)
- University of Tsukuba (JP)
- Queensland University of Technology (AU)
- Lincoln University (NZ)
- Chubu University (JP)
- Kyoto University (JP)
- RIKEN BioResource Research Center (JP)
- Kazusa DNA Research Institute (JP)
- ARC Centre of Excellence for Plant Success in Nature and Agriculture (AU)
- Research Organization of Information and Systems (JP)
- Nagoya University (JP)
Publication Details
- Journal
- The Plant Journal
- Published
- 2024-12-03
- DOI
- https://doi.org/10.1111/tpj.17178
- Citations
- 9
- Primary Topic
- Plant-Microbe Interactions and Immunity
- Type
- article
- Field-Weighted Citation Impact
- 5.40
Funders
- New Energy and Industrial Technology Development Organization
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency