EFFECTS OF INJECTABLE MINERAL AND VITAMIN SUPPLEMENTATION ON THE FERTILITY OF DAIRY COWS IN FTAI PROTOCOL

The study aimed to assess the impact of injectable mineral and vitamin supplementation, specifically Kit Adaptador®MIN and Adaptador®VIT (Biogénesis Bagó, Brazil), administered at the initiation of a fixed-time artificial insemination (FTAI) protocol, on the reproductive performance and fertility of Holstein cows. One hundred animals were divided into a non-supplemented control group (CG, n=50) and a treated group (TG, n=50). The TG received mineral and vitamin supplementation (1 mL/100kg live weight, IM) on the first day of the FTAI protocol (D0), along with intravaginal progesterone (P4) implantation and estradiol benzoate administration. Blood samples were collected at D0 and D17 to assess superoxide dismutase (SOD) enzyme activity and P4 levels. Ovarian and uterine ultrasound evaluations were conducted at D0 and D17, with follow-up exams performed 30 days post-FTAI for pregnancy diagnosis. Results showed a larger corpus luteum diameter in supplemented cows (CG=2.14±0.11 cm vs TG=2.64±0.11 cm, P=0.0037), along with increased SOD activity (CG=22% vs TG=25.36%, P=0.045). Moreover, there was a significant difference in pregnancy rates between groups (CG=18.18% vs TG=39.13%, P=0.0029). Injectable mineral and vitamin supplementation at the start of the FTAI protocol may impact post-ovulation corpus luteum development, enhance pregnancy rates, and stimulate essential endogenous antioxidant mechanisms in Holstein cows.