The Applicability and Limitations of the Spectrofluorometric Method for Determination of ALDH1 Activity in Serum and Plasma
The spectrofluorometric method has limited applicability for determining ALDH activity in plasma and serum. HPLC can measure ALDH1 activity in plasma or serum; however, factors like hemolysis and elevated liver enzymes significantly affect activity and must be considered in diagnostic interpretations. To enhance research quality on ALDH1 as a biomarker for diseases, including cancers, we recommend using control samples, reference materials, and purifying commercially available aldehyde substrates to improve method sensitivity.
Authors
- Renata Wolinowska (ORCID: https://orcid.org/0000-0002-2338-556X)
- Sylwia Michorowska (ORCID: https://orcid.org/0000-0001-7079-7527)
- Agnieszka Wiśniewska (ORCID: https://orcid.org/0000-0002-0137-8123)
- Joanna Giebułtowicz (ORCID: https://orcid.org/0000-0002-2567-4453)
- Piotr Wroczyński (ORCID: https://orcid.org/0000-0001-7494-2154)
Institutions
- Medical University of Warsaw (PL)
Publication Details
- Journal
- Diagnostics
- Published
- 2024-12-03
- DOI
- https://doi.org/10.3390/diagnostics14232721
- Citations
- 1
- Primary Topic
- Sulfur Compounds in Biology
- Type
- article
- Field-Weighted Citation Impact
- 0.29