Rapid and Safe Neutralization Assay for Circulating H5N1 Influenza Virus in Dairy Cows
Rapid and safe neutralization assays are required for highly pathogenic avian influenza viruses, including a clade 2.3.4.4b H5N1 subtype recently found in cows. Here, we report a neutralization assay using luminescent virus-like particles. This assay has lower biosafety requirements and provides a larger dynamic range than conventional methods. We applied this technique to evaluate the cross-reactivity of neutralizing antibodies induced by clade 2.3.4.4b candidate vaccine viruses (CVVs) with the cow-derived H5N1 virus. Our findings indicate that these CVVs share antigenic characteristics with the cow-derived H5N1 virus, suggesting the potential efficacy of vaccines developed using these CVVs.
Authors
- Fumitaka Momose (ORCID: https://orcid.org/0000-0003-3473-5809)
- Akihide Ryo (ORCID: https://orcid.org/0000-0001-6028-8091)
- Kaori Sano (ORCID: https://orcid.org/0000-0002-4887-1302)
- Takashi Okura (ORCID: https://orcid.org/0000-0002-6019-958X)
- Hideki Asanuma
- Kei Miyakawa (ORCID: https://orcid.org/0000-0002-2219-6839)
- Tomoko Arita
- Hideki Hasegawa (ORCID: https://orcid.org/0000-0002-6558-2297)
- Yasushi Suzuki (ORCID: https://orcid.org/0000-0002-1952-9604)
- Masayuki Shirakura
- Shinji Watanabe
- Noriko Kishida (ORCID: https://orcid.org/0000-0003-4501-568X)
- Makoto Ota
Institutions
- National Institute of Infectious Diseases (JP)
- Yokohama City University (JP)
Publication Details
- Journal
- Influenza and Other Respiratory Viruses
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1111/irv.70048
- Citations
- 3
- Primary Topic
- Influenza Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.16
Funders
- Japan Agency for Medical Research and Development
- Hokkaido University
- National Agriculture and Food Research Organization
- Kitasato University
- Centers for Disease Control and Prevention
- Japan Society for the Promotion of Science