Weizmannia coagulans BC99 Enhances Intestinal Barrier Function by Modulating Butyrate Formation to Alleviate Acute Alcohol Intoxication in Rats

<b>Background/Objectives:</b> Probiotics have great potential in improving acute alcohol intoxication. The aim of this study was to investigate the mitigating effect and mechanism of action of <i>Weizmannia coagulans</i> BC99 on acute alcohol intoxication (AAI) in SD rats. <b>Methods:</b> BC99 was divided into different doses administered by gavage to rats, and a rat model of acute alcohol intoxication was established by multiple gavages of excess alcohol. <b>Results:</b> Our study demonstrated that <i>W. coagulans</i> BC99 intervention significantly prolonged the latency period of intoxication; significantly attenuated alcohol-induced lipid elevation, liver injury, hepatic inflammation, and intestinal barrier damage; and lowered plasma endotoxin (LPS) levels in rats. In addition, <i>W. coagulans</i> BC99 could effectively restore the balance of intestinal flora, increase the abundance of <i>Lachnospiraceae</i>_<i>NK4A136</i>, <i>Prevotellaceae</i>_<i>NK3B31</i>, <i>Parabacteroides</i>, and <i>Ralstonia</i>, and thus increase the content of intestinal short-chain fatty acids (SCFAs), especially butyric acid. Moreover, we demonstrated through sodium butyrate validation experiments that butyrate could attenuate intestinal barrier damage and reduce the diffusion of LPS, thereby reducing liver inflammation. <b>Conclusions:</b> In conclusion, <i>W. coagulans</i> BC99 ameliorates acute alcohol intoxication in rats by increasing the abundance of butyrate-producing genera and thereby increasing butyrate abundance to alleviate intestinal barrier injury.