Protocol for whole-cell patch-clamp assay for HBV-mediated sodium influx in THP-1 macrophages
Sodium influx induced by hepatitis B virus (HBV) enhances activation of the NLRP3 inflammasome, in synergy with potassium efflux signals in macrophages. Here, we present a protocol for conducting patch-clamp assays to measure HBV-mediated sodium influx in THP-1 macrophages. We describe steps for differentiating, resting, and activating THP-1 macrophages and then outline the procedures for whole-cell patch-clamp recordings. For complete details on the use and execution of this protocol, please refer to Wang et al.<sup>1</sup>.
Authors
- Xiao-Juan Xin
- Yadi Tan (ORCID: https://orcid.org/0000-0002-5009-1057)
- Jingxue Wang
- Penghui Chen (ORCID: https://orcid.org/0000-0002-9655-490X)
Institutions
- Army Medical University (CN)
- The Affiliated Yongchuan Hospital of Chongqing Medical University (CN)
- Chongqing Medical University (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103462
- Citations
- 1
- Primary Topic
- Cannabis and Cannabinoid Research
- Type
- article
- Field-Weighted Citation Impact
- 0.45