Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans
Our results suggest collection of human saliva for biomarker identification must be performed with extreme diligence. We propose application of RNAlater should be avoided and snap freezing of the collected saliva is recommended when joint protein and RNA quantification is the ultimate goal.
Authors
- Szabolcs Maar
- Ildikó Bock-Marquette (ORCID: https://orcid.org/0009-0002-1284-9696)
- Anikó Takátsy
- Lilla Czuni
- Tibor Mintál
- Ferenc Gallyas (ORCID: https://orcid.org/0000-0002-1906-4333)
- Szilard Rendeki
- Jørgen Kosberg Hassve
Institutions
- University of Pecs (HU)
Publication Details
- Journal
- Heliyon
- Published
- 2024-11-28
- DOI
- https://doi.org/10.1016/j.heliyon.2024.e40752
- Citations
- 1
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.24
Funders
- Hungarian Scientific Research Fund
- Nemzeti Kutatási, Fejlesztési és Innovaciós Alap
- National Research, Development and Innovation Office