Green synthesis of Au-Pt bimetallic nanoparticels using Croton caudatus Geisel leaf extract and their biological studies

Biosynthesis of Au-Pt alloy type nanorods are reported using Croton caudatus Geisel leaf extract. The synthesized nanorods are well characterized and their preliminary biological profiles are analyzed. Metal nanoparticles have a lot of interesting characteristics, and the field of medicinal applications may benefit significantly from them. In this investigation, gold-platinum (Au-Pt) bimetallic nanoparticles were synthesized employing leaf extract from Croton Caudatus Geisel. Utilizing transmission electron microscopy (TEM) examination and powder X-ray diffraction, the size of the synthesized nanoparticles was found to be between 12 and 33 nm. Using Debye Scherrer equation, particle size of the synthesized nanoparticle is determined and also consistent with transmission electron microscopy (TEM) analysis. The materials tend to be rectangular-shaped. This is a single-step, green protocol that does not employ any capping or reducing agents. The bio-active compounds in leaf extract act as reducing/capping/stabilizing agents and also fabricated with surface of the nanoparticles. Since green synthesis is an easy, stable, quick, low-cost, and environmentally friendly process, it opens up new possibilities for the synthesis of nanoparticles. Bimetallic nanoparticle synthesis generally involves mixing two different aqueous metal solutions with a natural reducing agent, like a plant extract. It has been proposed that plant phytochemicals with reducing or antioxidant properties reduce metal ions into metal nanoparticles. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray (EDX) analysis, Fourier-transform infrared (FT-IR) spectroscopy, and ultraviolet (UV) spectroscopy were all utilized to completely characterize the synthesized nanoparticles. It has been found that biological studies exploring in vitro antibacterial, in vitro antifungal, and anti-cancerous activity showed significant implications. In accordance with study results, the synthesized nanomaterials had a strong cytotoxic effect on HeLa cancer cell lines, with an MTT assay IC 50 value of 37.17 μg/ml/24 h.