Biopanning Data Bank 2023: Updating and New Findings

Background: Biopanning, or phage display technology, has gained considerable research attention for discovering peptides, and antibodies, and understanding protein interactions, which are crucial for developing targeted therapeutics. The Biopanning Data Bank (BDB, http://i.uestc.edu.cn/bdb) serves as a repository for peptide biopanning results. However, its last significant update was in 2018, highlighting a research gap that needs urgent attention. Objectives: This study aims to update BDB with the most recent data and enhance the identification of Target-Unrelated Peptides (TUPs). Methods: A search of PubMed was conducted for recent articles related to "phage display" published between January 2018 and May 2023. Relevant data were manually curated and added to BDB. Each peptide’s target was identified using MimoSearch, while TUPScan was used to detect new TUPs. Results: As of October 2023, BDB contains 3,682 biopanning datasets from 1,771 papers. These datasets included 124 NGPD datasets and 3,558 conventional biopanning datasets, featuring 34,078 peptide sequences, 593 templates, 2,231 targets, 524 peptide libraries, and 324 crystal structures. Our analysis identified 1,110 possible TUPs and 60 highly reliable TUPs, including 26 novel discoveries. Conclusion: This update addresses critical research gaps by incorporating recent peptide data and introducing novel TUPs. BDB remains the most comprehensive resource for biopanning, playing a crucial role in peptide library research and supporting the development of new TUP predictors and mimotope decoding tools.