Quadrant darkfield for label-free imaging of intracellular puncta
QDF imaging enhances the study of subcellular structures in living cells, offering improved quantification of organelle content compared with darkfield without labels. This method can be simultaneously performed with other techniques such as quantitative phase imaging to generate a multidimensional picture of living cells in real-time.
Authors
- Thomas A. Zangle (ORCID: https://orcid.org/0000-0001-5899-3517)
- Tarek E. Moustafa (ORCID: https://orcid.org/0000-0001-5282-8246)
- R.L. Belote (ORCID: https://orcid.org/0000-0002-3001-0002)
- Edward R. Polanco (ORCID: https://orcid.org/0000-0001-7388-962X)
- Robert L. Judson-Torres
Institutions
- University of Utah (US)
- Huntsman Cancer Institute (US)
- The Ohio State University (US)
Publication Details
- Journal
- Journal of Biomedical Optics
- Published
- 2024-11-29
- DOI
- https://doi.org/10.1117/1.jbo.29.11.116501
- Citations
- 1
- Primary Topic
- Digital Holography and Microscopy
- Type
- article
- Field-Weighted Citation Impact
- 0.34
Funders
- Huntsman Cancer Institute
- National Institutes of Health
- National Cancer Institute
- Congressionally Directed Medical Research Programs