Determining Kinetics Parameters of Oxidase-Like Ce/Fe-Bimetallic Nanozymes Toward 3,3′,5,5′-Tetramethylbenzidine Oxidation

Herein, kinetics parameters, Km and Vmax, of oxidase-like Ce/Fe-bimetallic nanozymes toward 3,3′,5,5′-tetramethylbenzidine oxidation were determined. The Michaelis–Menten kinetic model was used for the evaluation of the kinetic of the nanozymemediated oxidation of 3,3′,5,5′-tetramethylbenzidine. To provide the quantitative and accurate values of kinetics parameters, Km and Vmax, the linear plot of Lineweaver–Burk was constructed. The results exhibited a Vmax as high as 67.56 nM min-1 for the oxidase-like Ce/Fe-bimetallic nanozymes. Besides, Km was found to be as low as 0.06 mM for the as-prepared nanozymes, revealing the high affinity of the nanozymes toward 3,3′,5,5′-tetramethylbenzidine. Moreover, the ratio Vmax/Km was estimated as a reliable index of catalytic efficiency of the nanozymes, revealing a high value of 1.0×10-3 min-1.