Engineering the secretome of Aspergillus niger for cellooligosaccharides production from plant biomass
Tailored secretome use increased COS/glucose ratio during sugarcane biomass degradation showing that deleting some enzymatic components is an effective approach for producing customized enzymatic cocktails. Our findings highlight the plasticity of fungal genomes as enzymes that target minor components of plant cell walls, and alternative cellulases were produced by the mutant strain. Despite deletion of important secretome components, fungal growth was maintained in plant biomass.
Authors
- Fernanda Lopes de Figueiredo (ORCID: https://orcid.org/0000-0001-7697-2234)
- Jaqueline Aline Gerhardt (ORCID: https://orcid.org/0000-0001-7443-6390)
- Telma Teixeira Franco (ORCID: https://orcid.org/0000-0002-9611-4541)
- Natália Sayuri Wassano (ORCID: https://orcid.org/0000-0003-2743-4549)
- André Damásio (ORCID: https://orcid.org/0000-0001-9304-3998)
- Fabiano Jares Contesini (ORCID: https://orcid.org/0000-0001-9436-1226)
- Uffe Hasbro Mortensen (ORCID: https://orcid.org/0000-0002-7794-7273)
- César Rafael Fanchini Terrasan (ORCID: https://orcid.org/0000-0002-7988-4755)
- Sarita Cândida Rabelo (ORCID: https://orcid.org/0000-0002-3153-7674)
- Everton Paschoal Antoniel (ORCID: https://orcid.org/0000-0002-8951-5531)
- Ana Corrêa (ORCID: https://orcid.org/0000-0003-3151-0735)
- Lucas Levassor
Institutions
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Universidade Estadual Paulista (Unesp) (BR)
- Technical University of Denmark (DK)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2024-11-29
- DOI
- https://doi.org/10.1186/s12934-024-02578-9
- Citations
- 3
- Primary Topic
- Biofuel production and bioconversion
- Type
- article
- Field-Weighted Citation Impact
- 0.55
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Universidade Estadual de Campinas