Administration of Delphinidin to Improve Survival and Neurological Outcome in Mice After Cardiac Arrest and Resuscitation

Reactive oxygen species (ROS) play an important role in ischemia-reperfusion (I/R) after cardiac arrest and cardiopulmonary resuscitation (CA-CPR). Early administration of vitamin C at a high dose in experimental models resulted in less myocardial damage and had a positive effect on survival after resuscitation. Here, we postulated that the ROS scavenging activity of an anthocyanin (i.e., delphinidin) would positively influence resuscitation outcomes. We hypothesized that administration of delphinidin immediately after CA-CPR could attenuate systemic inflammation in a standardized mouse model and thereby improve survival and long-term outcomes. Outcomes up to 28 days were evaluated in a control group (saline-treated) and a delphinidin-treated cohort. Survival, neurological and cognitive parameters were assessed. Post-CPR infusion of delphinidin deteriorated survival time after a 10 min CA. Survivors amongst the controls showed significantly more anxious behavior than in the pre-CPR phases. This tendency was also observed in the animals treated with delphinidin. In our study, we did not find an improvement in survival with delphinidin after CA-CPR and observed no effect on learning behavior. Our long-term behavioral tests clearly show that CA-CPR is associated with the development of post-interventional anxiety-like symptoms. Our findings open up scopes to investigate the intrinsic factors (e.g., oxidative stress, inflammatory and systemic-microbial response, etc.) influencing the therapeutic efficacy of anthocyanins in vivo.