The effect of <em>Lactiplantibacillus plantarum</em> and <em>Lacticaseibacillus rhamnosus</em> cell-free supernatant on mature polymicrobial biofilm mass of <em>Candida albicans</em> and <em>Escherichia coli&

Biofilm is one of the leading causes of prolonged infection known currently with higher treatment failure if caused by polymicrobial biofilm. Lactobacillus strain is known to produce antibiofilm substances that could disrupt biofilm by fungi and bacteria. This study aims to evaluate two probiotic strains, L. plantarum and L. rhamnosus’ ability as single and multistrain to disrupt the biofilm of Candida albicans and Escherichia coli. This study employed a true experiment post test only control group design, using C. albicans and E. coli clinical isolate as subjects. The subjects in this study are divided into control and treatment groups. Treatment groups were subdivided into those receiving L. plantarum, L. rhamnosus and both probiotics for 24 h. Pathogens were incubated for 48 h in a 96-well microplate to reach a mature state before treatment groups were exposed to probiotics suspension for 24 h. Then, biomass was monitored using Crystal Violet (CV) staining and read using an ELISA reader to obtain the Optical Density (OD) values. The biofilm mass between treatments and control groups demonstrated a meaningful difference (p < 0.05), further post hoc analysis demonstrated multistrain cell-free supernatant of L. plantarum and L. rhamnosus demonstrated the highest antibiofilm between treatment groups in lowering biomass of single and dual-species biofilm C. albicans and E. coli (C. albicans = 0.215 ± 0.086; E. coli = 0.218 ± 0.099; dual-species biofilm = 0.123 ± 0.025). With the exposure of 24 h, the combination of L. plantarum and L. rhamnosus can decrease the biomass of single or dual-species biofilm formed by C. albicans and E. coli.