Biosynthesis of sakuranetin regulated by <scp>O</scp>s<scp>MPK</scp>6‐<scp>O</scp>s<scp>WRKY</scp>67‐<scp>O</scp>s<scp>NOMT</scp> cascade enhances resistance to false smut disease
Rice false smut disease, caused by the fungal pathogen Ustilaginoidea virens, significantly restricts both the production and quality of rice grains. However, the molecular mechanism underlying rice resistance against U. virens remain largely elusive. Transcriptome analysis of rice panicles infected with U. virens revealing the crucial role of genes involved in sakuranetin biosynthesis in conferring resistance to the pathogen. In vitro assays demonstrated that sakuranetin was most effective at inhibiting mycelial growth, spore germination, and host infection by U. virens. The expression of OsNOMT, the key enzyme in sakuranetin biosynthesis, is directly regulated by the transcription factor OsWRKY67. Furthermore, OsMPK6, a mitogen-activated protein kinase, interacts with and phosphorylates OsWRKY67, thereby modulating sakuranetin biosynthesis and resistance to U. virens. Moreover, the exogenous application of synthetic sakuranetin significantly reduces U. virens infection. Our findings reveal that the OsMPK6-OsWRKY67-OsNOMT signaling cascade plays a pivotal role in rice resistance to U. virens by regulating sakuranetin biosynthesis.
Authors
- Jinbiao Ma (ORCID: https://orcid.org/0000-0003-0426-3986)
- Keyi Huang (ORCID: https://orcid.org/0009-0007-5405-4038)
- Min Zhang (ORCID: https://orcid.org/0000-0001-5108-1275)
- Ravi Gupta (ORCID: https://orcid.org/0000-0001-5242-9528)
- Sun Tae Kim (ORCID: https://orcid.org/0000-0002-3853-1419)
- Shangyu Gao
- Clive Lo
- Lirong Wei (ORCID: https://orcid.org/0000-0002-2431-9580)
- Guosheng Xiong (ORCID: https://orcid.org/0000-0002-4312-1676)
- Jingni Wu (ORCID: https://orcid.org/0000-0002-4819-0347)
- Qin Gu (ORCID: https://orcid.org/0000-0002-3722-6375)
- Xi Chen (ORCID: https://orcid.org/0000-0002-5748-470X)
- Yiming Wang (ORCID: https://orcid.org/0000-0003-0513-9039)
- Shujing Li (ORCID: https://orcid.org/0000-0002-4275-7103)
- Huimin Guo (ORCID: https://orcid.org/0000-0001-9581-4557)
- Jing Zhao (ORCID: https://orcid.org/0000-0002-7031-3024)
- Jian Gao (ORCID: https://orcid.org/0000-0002-9404-8771)
- Chenjie Yu (ORCID: https://orcid.org/0009-0007-9387-8953)
- Dacheng Wang (ORCID: https://orcid.org/0009-0005-3525-7820)
Institutions
- Kookmin University (KR)
- Nanjing Agricultural University (CN)
- Jiangsu Academy of Agricultural Sciences (CN)
- Institute of Plant Protection (CN)
- Pusan National University (KR)
- University of Hong Kong (HK)
Publication Details
- Journal
- New Phytologist
- Published
- 2024-11-29
- DOI
- https://doi.org/10.1111/nph.20308
- Citations
- 13
- Primary Topic
- Plant Gene Expression Analysis
- Type
- article
- Field-Weighted Citation Impact
- 3.04
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- Government of Jiangsu Province
- Natural Science Foundation of Jiangsu Province
- National Key Research and Development Program of China
- Institute of Genetics