Green synthesis of silver nanoparticles using <i>Pupalia lappacea</i> L. (Juss) and their antimicrobial application

Abstract This work reports green synthesis of AgNPs using aqueous extract of Pupalia lappacea (L.) Juss. The synthesized AgNPs were characterized using UV–visible spectroscopy, powder X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy supported with energy dispersive X-ray, and transmission electron microscopy. UV–vis spectroscopy reveals a characteristic surface plasmon resonance absorption band at 420 nm. TEM results showed synthesis of spherical shaped AgNPs with an average particle size of 14.48 nm. The synthesized AgNPs exhibited excellent inhibition zones against Gram-positive bacteria Staphylococcus aureus (26.33 ± 0.88 mm), Bacillus Coagulans (12.33 ± 0.33 mm); and Gram-negative bacteria Sphingomonas (24.33 ± 0.67 mm), Shigella dysenteriae (24.33 ± 0.67 mm) and Salmonella typhimurium (12.67 ± 0.33 mm). Therefore, Pupalia lappacea mediated AgNPs can be used for clinical and medical applications.